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KMID : 0380019940090020157
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 2 p.157 ~ p.164
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High Yield Saponin Production by Mass Cultures of Ginseng Transformed Tissue
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Bai Dong-Gyu
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Abstract
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Hairy root clones of Panax ginseng were established by selection of some hairy roots formed on the leaf, stem and root segments transformed with Agrobacterium rhizogenes strain A,. The transformed roots grew well in MS medium under the dark condition. To confirm the transformation with Ri TDNA, dot blot hybridization and opine analysis were performed. Among four hairy roots induced from different part of ginseng, the HB3 hairy roots were examined for selection of high- saponin- producing clones. Four clones isolated from HB3 hairy root cultures displayed various phenotypes characterized by growth and total saponin content. Maximum growth was obtained for cultures of HB3-10 clone and the content of total saponin was 0.55 wt%. Howeve;higher amount of total saponin was obtained with HB3-2 clone cultures( 0.74 wt% ) in spite of lower growth. Dot blot hybridization confirmed the introduction of Ri-T-DNA in the plant genome. In the opine test, agropine and mannopine were detected from all hairy root clones.
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